In this protocol, TiO2 nanoparticle dispersions in relevant biological media are produced by following a series of steps applied to a stock TiO2 aqueous nanoparticle dispersion.
This protocol focuses on two representative media broadly used for in vitro assays: phosphate-buffered saline solution (PBS) and Dulbecco's Modified Eagle Medium containing 10 % fetal bovine serum (DMEM-FBS). In both cases, bovine serum albumin (BSA) is utilized as a stabilizing agent; series specific serum albumin has been demonstrated to function as a nonspecific biocompatible stabilizer. This protocol is proposed for the preparation of dispersions for generic acute bioassay applications; its use for chronic toxicity evaluations is beyond the scope of this work and should be validated by use of proper controls.
2.Principles and scope
4.Reagents, materials and equipment
5.Preparation of TiO2 nanoparticle dispersions
5.1. Dispersion in PBS
5.2. Dispersion in DMEM with 10 % FBS
6.1. Dispersion in PBS
6.2. Dispersion in DMEM with 10 % FBS